Experimental infection of layer hens with a human isolate of Brachyspira pilosicoli
نویسندگان
چکیده
منابع مشابه
Evaluation of PCR amplification from fixed caecal tissue to determine the infection of experimentally infected laying hens with Brachyspira pilosicoli
Avian intestinal spirochaetosis (AIS) is a condition arising from colonization of the caeca and colon of birds with anaerobic spirochaetes of the genus Brchyspira. The purpose of the present study was to evaluate PCR amplification from formalin-fixed, paraffin-embedded tissue method for detecting infected laying hens with Brachyspira pilosicoli with the same primers that have been used for det...
متن کاملNeither Hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli Type Strain Caused Diarrhoea in Early-weaned Pigs by Experimental Infection
A hippurate-negative biovariant of Brachyspira pilosicoli (B. pilosicolihipp-) is occasionally isolated in diarrhoeic pigs in Finland, often concomitantly with hippurate-positive B. pilosicoli or Lawsonia intracellularis. We studied pathogenicity of B. pilosicolihipp- with special attention paid to avoiding co-infection with other enteric pathogens. Pigs were weaned and moved to barrier facilit...
متن کاملevaluation of pcr amplification from fixed caecal tissue to determine the infection of experimentally infected laying hens with brachyspira pilosicoli
avian intestinal spirochaetosis (ais) is a condition arising from colonization of the caeca and colon of birds with anaerobic spirochaetes of the genus brchyspira. the purpose of the present study was to evaluate pcr amplification from formalin-fixed, paraffin-embedded tissue method for detecting infected laying hens with brachyspira pilosicoli with the same primers that have been used for det...
متن کاملPotential for Zoonotic Transmission of Brachyspira pilosicoli
Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 12, No. 5, May 2006 869 6. Koprowski H. The mouse inoculation test. In: Meslin FX, Kaplan MM, Koprowski H, editors. Laboratory techniques in rabies. Geneva: World Health Organization; 1996. p. 80–7. 7. Orciari LA, Niezgoda M, Hanlon CA, Shaddock JH, Sanderlin DW, Yager PA, et al. Rapid clearance of SAG-2 rabies virus from dogs after oral vac...
متن کاملPCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces.
Previously-developed PCR protocols specific for the 16S rRNA gene of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli were adapted for the detection of these species in human faeces, following DNA extraction and purification using mini-prep columns. The limits of detection in seeded faeces for B. aalborgi and B. pilosicoli respectively were 2x10(2) and 7x10(3) cells p...
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ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 2003
ISSN: 0022-2615,1473-5644
DOI: 10.1099/jmm.0.05040-0